| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.066346 |
| Chromosome: | chromosome 3 |
| Location: | 5212106 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181750 | intron | ||
| lncRNA_TCONS_00234913 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAACACCCTTTCCCCCAACGCACAGGCAAGGCGGTCTGCGGATCCTGGT |
| Internal bar code: | ATGCGGGTGGGTTCCATAACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 52 |
| LEAP-Seq percent confirming: | 2.04082 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCGAGCAGACACAGATGT |
| Suggested primer 2: | GGCTTGCTCCGAGTACTTGT |