| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.066348 |
| Chromosome: | chromosome 1 |
| Location: | 7591542 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g800139 | (1 of 1) IPR026741//IPR026937//IPR027417 - Protein strawberry notch // Strawberry notch, helicase C domain // P-loop containing nucleoside triphosphate hydrolase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGACGCGGCGGCGATGGACGCGGCGACGGCCATGGACGCGCAGCAGC |
| Internal bar code: | GAGATAGCGGTTACCTATCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1281 |
| LEAP-Seq percent confirming: | 57.1429 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCATGTCGTCGTCGTCATC |
| Suggested primer 2: | GTCTGGAGGACCTGGAGGAT |