| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.066374 |
| Chromosome: | chromosome 5 |
| Location: | 2778159 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240850 | THIC1,THIC,THI8 | (1 of 1) 4.1.99.17 - Phosphomethylpyrimidine synthase; Hydroxymethylpyrimidine phosphate synthase | 3'UTR |
| Cre05.g240900 | (1 of 1) IPR000001//IPR024079 - Kringle // Metallopeptidase, catalytic domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTGTGATGGTTGTGTTCAAGCGAAACAGCGGTGCAGCCGGACCACAG |
| Internal bar code: | CCATTAGCAGGCGTTATCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1590 |
| LEAP-Seq percent confirming: | 98.4375 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGGGCAATGGGGATAGA |
| Suggested primer 2: | CATCAAACGCAAGCACCACA |