Insertion junction: CLIP2.066393_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.066393
Chromosome:chromosome 1
Location:4309354
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g029300 TPI1,TPIC1,TPIC (1 of 1) 5.3.1.1 - Triose-phosphate isomerase / Triosephosphate mutase; Triose phosphate isomerase, chloroplast precursor 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTGGACACGAGCTAGCGGAGGTGGATTGACAGGATGTTTCGCCGGGGT
Internal bar code:CGATTCTCCCGTATTGGGACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2390
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:54
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:GCCAACATCGAATTCGGCTC
Suggested primer 2:CGTAACGATCTGCAACGCTG