| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.066407 |
| Chromosome: | chromosome 7 |
| Location: | 1291375 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322176 | (1 of 1) K03002 - DNA-directed RNA polymerase I subunit RPA2 (RPA2, POLR1B) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACATTACTCGCACGTCGCAATTGCGGGCGTGAATTGGCGGAGTGGCACA |
| Internal bar code: | AACAAGTTCGGGGAACGTTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 987 |
| LEAP-Seq percent confirming: | 94.0 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTCAAGCCCGATCAAGT |
| Suggested primer 2: | TCTGAGGGGATGTTAGGGGG |