| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066416 |
| Chromosome: | chromosome 2 |
| Location: | 2963240 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095294 | (1 of 1) PTHR14187//PTHR14187:SF51 - ALPHA KINASE/ELONGATION FACTOR 2 KINASE // SUBFAMILY NOT NAMED | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGCCCCGATGATGGTCAGGTTCCACATTCTTGGGCTGTTGACGCCAC |
| Internal bar code: | TCCACATTGAATTGATAGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3941 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCAGAACGCGTCACAAC |
| Suggested primer 2: | ATCTTTTCTGAGCGCTGCCT |