| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.066422 |
| Chromosome: | chromosome 9 |
| Location: | 1022023 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402200 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCATCCATCTAGCCAGCCACTCGCATGCGAAAAGGCAGACGACACCTAC |
| Internal bar code: | AAGTTCGGCTAAGGATTAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 406 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGTGTTTGCTGCTTTCT |
| Suggested primer 2: | TCGAGTCGTGTCTGTGTGTG |