| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066423 |
| Chromosome: | chromosome 9 |
| Location: | 5614189 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTTGTAAGGCTGGAGGACTCGTGCCCAAAGACCCCTTGCACACGTCCC |
| Internal bar code: | TCCTCCCGTGAGACGGTGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3145 |
| LEAP-Seq percent confirming: | 95.0617 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAACCAAGCTGGACCACG |
| Suggested primer 2: | TTCTCCCTCTCCTGCCTACC |