Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.066431 |
Chromosome: | chromosome 10 |
Location: | 1863354 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g431100 | OPR126 | OctotricoPeptide Repeat protein 126 | outside_mRNA |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTTGAGGCTTGAGATAGATTTTCGCTCGTGGAGGGGCTATGGCAACTC |
Internal bar code: | GAAATCTGTCGAAGCAGTAAAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1848 |
LEAP-Seq percent confirming: | 78.7234 |
LEAP-Seq n confirming: | 37 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCTTCCAGTTGTCCCTTCC |
Suggested primer 2: | GCTGATGATTGGCAGGCATG |