| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066436 |
| Chromosome: | chromosome 12 |
| Location: | 1620265 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486950 | CSV7 | Chlamydomonas-specific family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACCATTTGGATCCGCTGTTTGCAACAACAATGTCAAACAACCCACAAT |
| Internal bar code: | TCAGTGGTCTGAACGTAACGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5422 |
| LEAP-Seq percent confirming: | 88.5057 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGTGGTCAGTGCCTAGG |
| Suggested primer 2: | GTGCGGCAAGTTGTGAATGT |