Insertion junction: CLIP2.066443_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.066443
Chromosome:chromosome 9
Location:430910
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre18.g749547 (1 of 1) PF07510 - Protein of unknown function (DUF1524) (DUF1524) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGCTTGGAATCTCAGGAAATTCATGTCACTACGATAGATAACATAAAA
Internal bar code:GTCTTATGCACCAAGTCGCAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:4736
LEAP-Seq percent confirming:98.3333
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:ATTAGCTGGCGAGTCCGATG
Suggested primer 2:CAGTAAACGTGGCCCTTCCT