| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066444 |
| Chromosome: | chromosome 8 |
| Location: | 1706126 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g366250 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGGTACACATGCTACGCTGTGCTGCCGTCATCTAAACATTCCTGACGC |
| Internal bar code: | TTGATTATCGTCAAACATCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1623 |
| LEAP-Seq percent confirming: | 62.0 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTTCAAGGCCATGGCTAA |
| Suggested primer 2: | CAGGTACTAGGTGTTGGCCG |