| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066445 |
| Chromosome: | chromosome 11 |
| Location: | 4412867 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g483100 | (1 of 1) IPR019931 - LPXTG cell wall anchor domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACCACAGCACCGCCGCAAAGGAGTAGATGTCCTGTGAGGGCCAGCAAA |
| Internal bar code: | TTAGGAGTAAAGGCAGTCAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5416 |
| LEAP-Seq percent confirming: | 86.9565 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGTTGGAGCTGTTCACG |
| Suggested primer 2: | TCCCGCTCTAGCCTTTGTTG |