Insertion junction: CLIP2.066453_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.066453
Chromosome:chromosome 2
Location:6859469
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g388504 (1 of 16) IPR003903 - Ubiquitin interacting motif 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAATCACACATAGGAGGATCCCATACTGGCGGTTAGTAACAAACCTCC
Internal bar code:GGTTTGCCAAAGTGTGTCTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:6043
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGACGGGAAAGCAAGGAG
Suggested primer 2:GAATCATTGTCATGCGCCCC