| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066475 |
| Chromosome: | chromosome 1 |
| Location: | 3501705 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022800 | FAL1 | Similar to Flagellar Associated Protein FAP159 | 5'UTR |
| Cre01.g022850 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGAAAAATAATTTATACACAAATGAAGTAACAGTTTCGCTAAAGGGGG |
| Internal bar code: | TACGAGAACGTTTTTTAACCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 881 |
| LEAP-Seq percent confirming: | 5.88235 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGACATAGGGCGCTAATG |
| Suggested primer 2: | GGTGTCAAATATTCGGCCGC |