Insertion junction: CLIP2.066482_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.066482
Chromosome:chromosome 2
Location:4880260
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g109683 (1 of 3) IPR002912//IPR011598 - ACT domain // Myc-type, basic helix-loop-helix (bHLH) domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATATGAAGTGAAGTGCCGACGTCCAGAGGTACAGCGGGGGGGGGGGGCA
Internal bar code:TGAAATACTGTCAGGAAAACAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1820
LEAP-Seq percent confirming:96.5517
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCGTTGTTGGTGTTGCTG
Suggested primer 2:TAAACAAAGTGCAACCCGCG