| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.066488 |
| Chromosome: | chromosome 12 |
| Location: | 2282244 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508250 | (1 of 1) PTHR22975:SF9 - F6D8.33-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCATTTTGCTGCCTCCCTAGTACCATATACATAGAGTCACGCGTCTG |
| Internal bar code: | GTTTACTCGCGGTATCTACGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1232 |
| LEAP-Seq percent confirming: | 54.5455 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTCCGGTGATCAGCTAGC |
| Suggested primer 2: | CTTACTGTGAAGCGCGATGC |