Insertion junction: CLIP2.066492_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.066492
Chromosome:chromosome 11
Location:4201554
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g481600 GAS28 Hydroxyproline-rich glycoprotein, stress-induced; (1 of 71) PF12499 - Pherophorin (DUF3707) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAAAGAGTCGCCACCAACCCCCCGAACAACTGAAATTGCGCGCCGAAA
Internal bar code:TGGGATAGCACCACTTATCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2301
LEAP-Seq percent confirming:98.0
LEAP-Seq n confirming:49
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:CACATACATGGCCTTGGGGT
Suggested primer 2:ATGGAAAGTAGTTGGGGGCG