| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.066495 |
| Chromosome: | chromosome 10 |
| Location: | 835172 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423350 | (1 of 1) PF07052 - Hepatocellular carcinoma-associated antigen 59 (Hep_59) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGGAGGGTGTGCTGTATCGACACTGGAGGCAGAGGCGGGAGGCGGGCG |
| Internal bar code: | AAGTGGTCGATAGACCACTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2403 |
| LEAP-Seq percent confirming: | 88.0 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATCTGGATTTGCGCGGTC |
| Suggested primer 2: | TTGGCAGTCCTGGGTTTGTT |