Insertion junction: CLIP2.066506_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.066506
Chromosome:chromosome 6
Location:1353221
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre06.g259350 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGTGACCCAACCATGTCACGAAGCCAGGGAGACGACGCGGTCATGTGG
Internal bar code:GAAGGTTTAACATCGTGATGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:178
LEAP-Seq percent confirming:8.33333
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACACAACATCGCACACG
Suggested primer 2:CACACGCACGCAGTATGATG