Insertion junction: CLIP2.066524_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.066524
Chromosome:chromosome 9
Location:4136475
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g399439 (1 of 93) 6.3.2.19 - Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGACAGCGCGTCTGCTACTGTCACCTGTCATGTCTGCTACAACCTCCC
Internal bar code:GCCGGGGTGAGACAGACCGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:183
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:AGGATGAGGCGTTGATAGCG
Suggested primer 2:GGAAATTTGATCGGAGCCGC