| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066531 |
| Chromosome: | chromosome 14 |
| Location: | 1606595 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g618550 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCGGGCAGCGCCGGCGGCGCTGGCGGCTTCGCTGCCGCCATCACTGC |
| Internal bar code: | CACTCGACATAACGTTGTTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 88 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTCTGCAGGTAACGTGGG |
| Suggested primer 2: | GGTTGTGGATGTGTGGTTGC |