| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.066535 |
| Chromosome: | chromosome 14 |
| Location: | 3589751 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630895 | (1 of 1) PTHR13789:SF90 - FAD-BINDING MONOOXYGENASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAAGTTTGGGCTGGGATTTACAACCTGCCTCCCTCCCCCGCCCCCAAG |
| Internal bar code: | ATGCACCTTCTCAGTTAAACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 54 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTACTCTGTCGCTGCTGCT |
| Suggested primer 2: | TCTGGCCTGTGTGTGTGTTT |