Insertion junction: CLIP2.066537_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.066537
Chromosome:chromosome 3
Location:2318532
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g157950 (1 of 1) IPR011989//IPR011990//IPR016024 - Armadillo-like helical // Tetratricopeptide-like helical domain // Armadillo-type fold 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATAAGCACACAGCGCGGCTTTGGAACTCGCCGGTCTACCGCTGTCTTG
Internal bar code:TTTGTCAGGCGGCGTAGCTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1516
LEAP-Seq percent confirming:95.6522
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CCTACACATGCTGCACAGGA
Suggested primer 2:CCTGCGCGAGAATTAAGTGC