Insertion junction: CLIP2.066542_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.066542
Chromosome:chromosome 13
Location:3850446
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGAATTCCTCACGAGCCGCGGCGCGGGGCTTGACCTTGGTCACGACCT
Internal bar code:TGTGCGGCTCGAGAAAACGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3584
LEAP-Seq percent confirming:99.1667
LEAP-Seq n confirming:119
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:120

Suggested primers for confirmation by PCR

Suggested primer 1:CTACATGCAAACGAGCGCTC
Suggested primer 2:CAGGCAAGCAGCTAGGAACT