| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.066561 |
| Chromosome: | chromosome 4 |
| Location: | 1948325 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218750 | THB4 | Truncated hemoglobin; (1 of 1) IPR012292 - Globin/Protoglobin | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATGGGTGCGTGCCGAACAACGTCTCATCATGGCCATCCAGGCGCGAAG |
| Internal bar code: | GTATTCTCTAGAATCATATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2568 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGCTGGCAAGGCAGAGTC |
| Suggested primer 2: | AAAGGTCCTTCAGCACCTCG |