| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066564 |
| Chromosome: | chromosome 13 |
| Location: | 1893000 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575750 | (1 of 1) 2.5.1.105 - 7,8-dihydropterin-6-yl-methyl-4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate synthase / Dihydropteroate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAATCTCAACGTAGCTTGGTCGGGTAGCGTGCCGTTGCACCATGTGCG |
| Internal bar code: | CACTAACCCGGTGGTACTGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1279 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATTGTGCTTTGACCCAGC |
| Suggested primer 2: | GCCGCTAATCGTCGTGAAAC |