| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.066564 |
| Chromosome: | chromosome 13 |
| Location: | 1893006 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575750 | (1 of 1) 2.5.1.105 - 7,8-dihydropterin-6-yl-methyl-4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate synthase / Dihydropteroate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGCATTGGTTACAAGAGGGACGTTGAGAACGTTTGCCGAGGTGTACCA |
| Internal bar code: | TTTCATACGGGAGTGTGGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2817 |
| LEAP-Seq percent confirming: | 98.6667 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGCTAATCGTCGTGAAAC |
| Suggested primer 2: | CCATTGTGCTTTGACCCAGC |