| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.066582 |
| Chromosome: | chromosome 1 |
| Location: | 6810188 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048800 | NAT8 | (1 of 36) PF00583 - Acetyltransferase (GNAT) family (Acetyltransf_1); N-acetyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCAACATGGTGGAGGCACGAACCGGCGGATGTGGATCCCTTGGAACTC |
| Internal bar code: | TGTAGCCATAAAAATATTCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 758 |
| LEAP-Seq percent confirming: | 36.3636 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGCATCGTTCTATGAGGA |
| Suggested primer 2: | TGCAAGGCAAGCTTCGTTTC |