| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066598 |
| Chromosome: | chromosome 16 |
| Location: | 5583264 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681500 | (1 of 1) K07002 - uncharacterized protein (K07002) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAATCGAAACGGGCCTTGAGACAATCTGCGCTTGTCGGGCCGCACATG |
| Internal bar code: | AAGTACCACTCCTCTCAAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2368 |
| LEAP-Seq percent confirming: | 83.1169 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGAGTACTGCGCGCTTCA |
| Suggested primer 2: | GGCCACTTCCAAGACGAAGA |