Insertion junction: CLIP2.066605_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.066605
Chromosome:chromosome 2
Location:3187553
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g096737 (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease gene_edge/mRNA_edge/3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCACGCCCCGAAATTCCCCGGGACCCCCCTTGAGGGGACGACCGACCC
Internal bar code:TGTTAGTATTTCACTGTTGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3547
LEAP-Seq percent confirming:72.7273
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTGACCAACACGCTGAAC
Suggested primer 2:TCCACCAAAACTTTGCCCCT