| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066610 |
| Chromosome: | chromosome 4 |
| Location: | 2472176 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221950 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATAAGCATGTGCAGTGGAATTAGGGGCTTCCGAGCAGCTACGTTATTTC |
| Internal bar code: | TTGGAGGTGGGGCAGAATCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1956 |
| LEAP-Seq percent confirming: | 87.7193 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTCGCCGCATTTACGAAG |
| Suggested primer 2: | GGGAAGAGAGGAGAGGGGAG |