Insertion junction: CLIP2.066616_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.066616
Chromosome:chromosome 6
Location:6392667
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g293362 (1 of 726) IPR011009 - Protein kinase-like domain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAGCGACTTCTGCGAGTTCGTTGGCGCGAGAACGCTTCATGATACTTGC
Internal bar code:TTGCGGCCGGTGTATGTGTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:946
LEAP-Seq percent confirming:94.5946
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:ATCGCACACTCGAGACCAAG
Suggested primer 2:AGCTACTACTTCCGCCTCCA