| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.066619 |
| Chromosome: | chromosome 12 |
| Location: | 3223693 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499800 | ASA10 | Mitochondrial F1F0 ATP synthase associated protein 10 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGTTCCATTGTCGCGTGTCTGGCGTGTCCCGATCCCAAGGCCAAGCCA |
| Internal bar code: | TGGGGGCCGATCTTCAGGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2156 |
| LEAP-Seq percent confirming: | 84.4828 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGGCACTAGATACAGGCC |
| Suggested primer 2: | TACCTGAACACGTTCACCGG |