| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.066621 |
| Chromosome: | chromosome 10 |
| Location: | 4098912 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448350 | (1 of 3) PTHR10361 - SODIUM-BILE ACID COTRANSPORTER | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACGTGGCATACGTACTGTTAAGGGGAAGAAGTCAAGGTTGCAGATAT |
| Internal bar code: | TTGCGTTAGTGATTGACAGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1579 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCCTGGTGTTTAGCCTA |
| Suggested primer 2: | AAAGAGGATTGACTGCCGCA |