Insertion junction: CLIP2.066623_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.066623
Chromosome:chromosome 16
Location:913218
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g648300 PHT6,PHT4-6,PHT4F Sodium-dependent phosphate transporter, major facilitator superfamily; (1 of 3) PTHR11662:SF243 - ANION TRANSPORTER 6, CHLOROPLASTIC-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTCCTGTGTGTGGCACAGCGACACCACACCCACACATCACTGCTGCCG
Internal bar code:CTTCCATCTATAGGTTAAAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:955
LEAP-Seq percent confirming:95.1219
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACCACCGACAAGCGTAT
Suggested primer 2:ATCGTTGCGTGTTGGAGTCT