Insertion junction: CLIP2.066627_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.066627
Chromosome:chromosome 12
Location:8564511
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g548900 ROC23,ROC112 (1 of 2) PTHR23181//PTHR23181:SF7 - RHO GTPASE ACTIVATING PROTEIN // SUBFAMILY NOT NAMED 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATTGGTGTGAATACACACTTAAACCCACAAGACAGTCCACAGAAAGTAT
Internal bar code:TCGCGGCCTTGCCTGAGGTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:1392
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CGACTGCCAGCGACTGATAT
Suggested primer 2:CACGCTTCATGGATCGCTTG