| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066637 |
| Chromosome: | chromosome 10 |
| Location: | 4153528 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448850 | FAP135,ODA5AK,CAL5 | (1 of 3) 3.4.22.54 - Calpain-3 / Muscle-specific calcium-activated neutral protease 3; Calpain-Like Flagellar Associated Protein 135 | 3'UTR |
| Cre10.g448900 | (1 of 80) IPR003882 - Pistil-specific extensin-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAGCACAAGGCCACCGGGCGTTTCAAGTTTTGCCAGCTAGAATGTCGC |
| Internal bar code: | ACCGCACCCAGACGCACAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 190 |
| LEAP-Seq percent confirming: | 2.94118 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGAGAATGTGCTCACCGT |
| Suggested primer 2: | TAGTGCCTGCCTACCAAAGC |