| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.066638 |
| Chromosome: | chromosome 5 |
| Location: | 213345 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g242950 | MRPL22,uL22m | (1 of 1) K02890 - large subunit ribosomal protein L22 (RP-L22, MRPL22, rplV); Mitochondrial ribosomal protein L22 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACATTCCATAGCCTCCTTTGCGATTTCACTTTTGGTAAGCGATCGAGGG |
| Internal bar code: | TTGTTGTCGAAAGTGCGCGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1166 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCCACCTATCCCCTTGA |
| Suggested primer 2: | TTGCAATGCAAGAAACGGGG |