| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.066643 |
| Chromosome: | chromosome 16 |
| Location: | 7509702 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682363 | (1 of 4) 2.4.1.229 - [Skp1-protein]-hydroxyproline N-acetylglucosaminyltransferase / transferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAACCCTGCCTCATTGCACCTAGTGCCCGCCTTGCACCTTCGGCAGCCA |
| Internal bar code: | GGTGGAGAAAGGGGCGTTTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 843 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACTTTGAGCACGACCAGA |
| Suggested primer 2: | TGCGGCGTTTGAGTAAGACT |