Insertion junction: CLIP2.066689_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.066689
Chromosome:chromosome 13
Location:2153115
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g577700 (1 of 100) IPR001810 - F-box domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAAAGCCGTGTCGCACATTACCACTCAAAACGCCGGAGCGGCGATGCCC
Internal bar code:GTCGTCGAGATAAGAAATGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1163
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTGATACGTTTCGTGCTG
Suggested primer 2:GAGCTTCTCTAGAGGGGCCT