| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066712 |
| Chromosome: | chromosome 12 |
| Location: | 5228147 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527400 | PGM9 | Phosphoglycerate mutase, 2%252C3-bisphosphoglycerate-independent; (1 of 2) 5.4.2.12 - Phosphoglycerate mutase (2,3-diphosphoglycerate-independent) / Phosphoglyceromutase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCAGTGGGGGTAGCGGCACGGGGTGCGCGTAGCTGTGATGTTGGGCAA |
| Internal bar code: | GTGGTATTAACTCGTGTAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 488 |
| LEAP-Seq percent confirming: | 14.2857 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAAGGGCAAGGCTTCAT |
| Suggested primer 2: | CAACACCACCTCAGTACCCC |