| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.066723 |
| Chromosome: | chromosome 5 |
| Location: | 2020220 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236500 | (1 of 1) 3.5.1.23 - Ceramidase / Acylsphingosine deacylase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCAAAGAAAACGAGCGGACCACAGGCAGAGGCGTTAGTTGGGGGGGAG |
| Internal bar code: | CCGATAGTAATGCTAAAACTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 338 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTTTGGGTTTGTGTACC |
| Suggested primer 2: | TCGCAAGGTACATATCGGCC |