| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066732 |
| Chromosome: | chromosome 12 |
| Location: | 9450411 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g540950 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGCGCTTCGGTCCGTGCCTAGCGCTGCATACAGCTAGATACTAATAC |
| Internal bar code: | TCCGGGGAAGAGTAGACGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3635 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGTCTCTGCAGCAGGTT |
| Suggested primer 2: | ACCCCTAGCAGCAGTGGATA |