Insertion junction: CLIP2.066736_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.066736
Chromosome:chromosome 10
Location:5560664
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTTGACTACTGTTTGCATGAACACCGTGTATGTCTACGCGACTGACTTG
Internal bar code:TGCGATGTATGGACCCGCTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2835
LEAP-Seq percent confirming:97.561
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:CACACTCGAAACGCAGGTTG
Suggested primer 2:GCCTTCCAGCAAACACACTG