Insertion junction: CLIP2.066737_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.066737
Chromosome:chromosome 6
Location:8767802
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g310950 FAO7 (1 of 1) 1.5.8.3 - Sarcosine dehydrogenase; FAD-dependent oxidoreductase 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAGTTCAAGTGTTGGCGGAGGTGTGGGAACTTGCTTGGAGGTGCCGAC
Internal bar code:AGTGGTTATAAACTGACATTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1832
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGCACGACAGCACATTTG
Suggested primer 2:AGGTTGCGAGTGGGAAGATG