Insertion junction: CLIP2.066738_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.066738
Chromosome:chromosome 7
Location:1046006
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g319800 (1 of 1) IPR000104//IPR009148 - Antifreeze protein, type I // Streptococcal non-M secreted SibA 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTTAGTGCCATGCGGCGTCTGGGTGATAGTTGGTTGGCGATCAGCGGA
Internal bar code:TTGCGAGAGATGATCCTGTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2341
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:CCACAACTTCGACTTGCGTG
Suggested primer 2:GCCCGCGCCATGTATTTTAG