Insertion junction: CLIP2.066738_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.066738
Chromosome:chromosome 7
Location:1046008
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g319800 (1 of 1) IPR000104//IPR009148 - Antifreeze protein, type I // Streptococcal non-M secreted SibA 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAATAACCAACTATCAACCGACGCCGCATTGCAATCAAGGCATTCATAAG
Internal bar code:ATTTGCATAATGTCGGTGAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:4460
LEAP-Seq percent confirming:95.3488
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCGCGCCATGTATTTTAG
Suggested primer 2:CCACAACTTCGACTTGCGTG