Insertion junction: CLIP2.066741_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.066741
Chromosome:chromosome 16
Location:4155203
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre16.g670550 XPG3 DNA repair 5'-3' exonuclease; (1 of 1) K15338 - flap endonuclease GEN [EC:3.1.-.-] (GEN1, GEN) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTGGGTCAGGACGGGGGTCCGACCGGGGAGCGTGGGCATGCTTTTCGC
Internal bar code:GGCGCTATTTCGTTTAGATGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:299
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTCTGAGTGTGGGCATGT
Suggested primer 2:TCCTCCTGACCTATCGCACA