Insertion junction: CLIP2.066747_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.066747
Chromosome:chromosome 10
Location:2109947
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g433050 ARCS,DRP5B,ARC5,DRP8 Putative dynamin GTPase involved in plastid division; (1 of 1) PTHR11566:SF78 - DYNAMIN-LIKE PROTEIN ARC5 3'UTR
Cre10.g433100 OBGE1,OBGE Factor possibly involved in assembly of the ribosomal 50S assembly; (1 of 1) K03979 - GTP-binding protein (obg) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTTCATGAATATGCGTACGGGGTGCACTGTGTTGCAGCACCTTGACCCA
Internal bar code:GATTGGGATTAAAATGCTCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:5553
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:81
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:81

Suggested primers for confirmation by PCR

Suggested primer 1:TTGATGCCCAAAATGCGGTG
Suggested primer 2:GCATAGCATGGCACGATGTG